Production of Metabolites in Microalgae Under Alkali Halophilic Growth Medium Using a Dissolved Inorganic Carbon Source

Abstract The production of metabolites in microalgae is influenced by extreme cultivation conditions. Bicarbonate an inorganic carbon source for phototrophic culturing. In this study, the effect dissolved (DIC) (0.4–13 g L −1 ) on accumulation Dunaliella tertiolecta biomass presented. highest levels primary [lipid (239.6 ± 24.3 mg ), protein (336.2 47.5 )], secondary [total phenolic (12.8 2.0 total flavonoid (14.4 2.3 ascorbate (4.7 1.1 and pigments [chlorophyll (27.2 3.1 carotenoid (2.0 0.1 )] were observed when cells grown with 5.7 DIC (NaHCO 3 . concentrations (1.5 obtained a mixture (4.3 + , NaHCO Na 2 CO ). This study recommends optimal bicarbonate maximising generation biomass. It also demonstrates that exogenous excessive growth medium would be effective stressor to produce high-value or alkali-halophilic strains. Graphical